Degree                  Institute/Board                                        Division                         Year

PhD                       Govt. College University, Lahore             1^st Div                             2017
(Biotechnology)
 
M.Phil.                  Govt. College University, Lahore             1^st Div                             2009
(Biotechnology)
M.Sc.                    Govt. College University, Lahore             1^st Div                             2006
(Botany)
B.Sc.                     University of the Punjab, Lahore              2^nd Div                            2004
(Zool, Bot, Chem)
F.Sc.                     B.I.S.E, Gujranwala                                  1^st Div                             2002
(Pre Med)
Matric                   B.I.S.E, Gujranwala                                  1^st Div                           1999
                             
B.Ed                     Allama Iqbal Open University                    1^st Div                           2006
Islamabad

GRE                      Scored 64 in GRE                                                                          2010

1. Four years doctoral research experience under the supervision of Muhammad Nauman Aftab at the institute of Industrial Biotechnology, Govt. College University, Lahore, Pakistan under the topic “Expression of GH13 family gene from Thermotoga petrophila into E. coli and characterization of the recombinant enzyme”.
2. Six months research experience in the project entitled Synthetic gene network for monitoring shear stress sensor activity in endothelial cells under the supervision of Rob Krams at the Bioengineering department, Imperial College, London.
3. One Year post graduate research experience under the supervision of Muhammad Nauman Aftab at the institute of Industrial Biotechnology, Govt. College University, Lahore under the topic Cloning and Expression of xylanase gene from Bacillus licheniformis into E.coli
4. One Year post graduate research experience under the supervision of Muhammad Mohsin javed at the institute of Industrial Biotechnology, Govt. College University, Lahore under the topic Ethnol Fermentation of Low Quality Dates by Yeast.

1. Muhammad Idrees, Zia Ud Din, Haleema Sadia, Asma Zafar, Shahab-ud-Din Kakar, Saeed Ahmed Essote, Ghulam Dastagir and Muhammad Zahid Mustafa, Effect of zinc bacitracin on various hematochemical and production attributes of broiler chickens. 2025. Japanese Journal of Veterinary Research 73(1): 1–9. IF: 0.4.
2. Asma Zafar, Ziaur Rahman, Hira Mubeen, Saira Sarwar, Samreen Fatima, Madood Makhdoom, Anosha Ashraf, Ghulam Sakina, Muhammad Nauman Aftab. Purification and characterization of a laccase enzyme produced by co-culturing of locally isolated Aspergillus niger and Aspergillus oryzae. 2025. Pak. J. Bot., 57(4): DOI: http://dx.doi.org/10.30848/PJB2025-4(13), IF: 1.2

3. Asma Zafar, Ammara Masood, Ziur Rahman, Attia Hamid, Mah Hoor Javed, Amna Zulfiqar, Samreen Fatima, Madood Makhdoom, and Muhammad Nauman Aftab, 2024. Cloning, purification, and functional characterization of recombinant pullulanase from Bacillus cereus ATCC 14579 for improved detergent performance. Journal of Surfactants and Detergents. HEC Category: X, IF: 1.972
4. Hira Mubeen, Ammara Masood, Asma Zafar, Zohaira Qayyum Khan, and Muneeza Qayyum Khan. Insights into AlphaFold’s breakthrough in neurodegenerative diseases. Irish Journal of Medical Science (1971-) (2024): 1-12. HEC Category: W, IF: 2.1
5. Asma Zafar, Ziaur Rahman, Attia Hamid, Fatima Sughra, Madood Makhdoom, Samreen Fatima, Haseeb Ahmad, Zeenat Mehmood, Maryam Khan, and Muhammad Nauman Aftab. Heterologous expression and characterization of a novel thermostable and alkali stable recombinant lipase enzyme from Bacillus thuringensis into coli BL21(DE3) for detergent formulation. Journal of Surfactants and Detergents. 2024;1–16. HEC Category: X, IF: 1.972
6. Nadia Iqbal, Ammara Masood, Hira Mubeen, Farah Deeba, Raheela Waheed, Iqra Noreen, Asma Zafar, Plosha Khanum. ATP Synthase Subunits: Structure and Role in Plants Under Stress Conditions. Pakistan Journal of Botany. 55(5). 2023. HEC Category: X. IF: 0.75

7.     Attia Hamid, Asma Zafar, Sabahat Latif, Liangcai Peng, Yanting Wang, Iram Liaqat, Muhammad Afzal, Ikram ul Haq, Ikram, Muhammad Nauman Aftab. Enzymatic hydrolysis of low temperature alkali pretreated wheat straw using
immobilized β-xylanase nanoparticles. RSC Advances, 13, 1434-1445. HEC Category: W, IF: 4.036, 2022.

8.     Asma Zafar, Ziaur Rahman, Hira Mubeen, Javairia Makhdoom, Javeria Tariq, Mahjabeen, Zulqurnain Ali, Attia Hamid, Eeza Shafiquea and Muhammad Nauman Aftab. Heterologous expression, molecular studies, and biochemical characterization of a novel alkaline esterase gene from Bacillus thuringiensis for detergent industry. RSC Advances, vol: 12, pages: 34482, HEC Category: W, IF: 4.036, 2022.

9.     A. Hamid, A. Zafar, I. Liaqat, M. S. Afzal, L. Peng, M. K. Rauf, I. ul Haq, A. ur-Rehman, S. Alia, M. N. Aftab. Effective utilization of magnetic nano-coupled cloned β-xylanase n saccharification process. RSC Advances, vol: 12, issue: 11, pages: 6463-6475, HEC Category: W, IF: 3.361.

10.  A. Zafar, A. Hamid, L. Peng, Y. Wang, M. N. Aftab, Enzymatic hydrolysis of lignocellulosic biomass using a novel, thermotolerant recombinant xylosidase enzyme from Clostridium clariflavum: a potential addition for biofuel industry, RSC Advances, vol: 12, pages: 14917-14931, HEC Category: W , IF: 4.036, 2022.

1. M. Q. Khan, H. Mubeen, Z. Q. Khan, A. Masood, A. Zafar, J. I. Wattoo, A. un Nisa, Computational insights into missense mutations in HTT gene causing Huntington’s disease and its interactome networks, Irish Journal of Medical Science, HEC Category: X , IF: 2.089, 2022
2. A. Zafar, S. Yousaf, M. N. Aftab, A. Hamid, J. I. Wattoo, A. Masood, H. Mubeen, Purification and characterization of a novel pullulanase enzyme from Bacillus thuringiensis for detergent industry, Revista Mexicana de Ingeniera Qumica, vol: 21 , issue: 1 , pages: 1-17, HEC Category: X , IF: 2.148, 2022
3. Saima Aftab, Attia Hamid, Mahjbeen, Javeria Tariq, Zulqurnain Ali, Asma Zafar, Cost-effective production of bioethanol from low-quality apples by using Saccharomyces cerevisiae, Biologia pakistan, vol: 67 , issue: II , pages: 119-124, 2022
4. Zia Ud Din, Muhammad Nauman Aftab, Nargis Haidar Kakar, Muhammad Zahid Mustafa, Asma Zafar, Irfana Iqbal, Irshad Ahmad, Simon. G. Patching, Muhammad Ali Khan, Tauseef M Asmat, Comparative Effects of Bacillus Probiotic and Antibiotic on the Growth Performance of Indigenous Aseel Chickens, International Journal of Agriculture and Biological Sciences, pages: 37-45, 2022

15.  Asma Zafar, Muhammad Nauman Aftab, Anam Asif, Ahmet Karadag, Liangcai Peng, Hassan Ufak Celebioglu, Muhammad Sohail Afzal, Attia Hamid and Irfana Iqbal. Efficient biomass saccharification using a novel cellobiohydrolase from Clostridium clariflavum for utilization in biofuel industry. RSC Advances. (2021). 11, 9246. DOI:10.1039/d1ra00545f. (Impact factor: 3.119)

16.  Attia Hamid, Zahid Hussain, Muhammad Tayyab, Asma Zafar, Muhammad Adnan Nawaz, Sikander Ali, Asad ur Rehman, Muhammad Nauman Aftab. Production and characterization of a thermostable extracellular esterase from Aspergillus niger. Revista Mexicana de Ingeniería Química. (2021). (Accepted). (Impact factor: 1.139).

17.  Komal Shamshad, Umer Farooq Gohar, Hamid Mukhtar, Asma Zafar and Shamroza Mubarik. High Performance Liquid Chromatography Method Development and an Innovative Technique for Validation of Artemether and Lumefantrine Oral Dry Suspension. Biomedical Journal of Scientific & Technical Research. (2020). DOI: 10.26717/BJSTR.2020.32.005234

18.  Muhammad Saleem, Syed Muhammad Ishaque, Shafi Muhammad Khosa, Zia Ud Din, Nargis Haider Kakar, Abdul Rauf, Muhammad Ali Khan, Asma Zafar, Irfana Iqbal. Line Probe Assay, The Best Molecular Diagnostic Tool, and Improve the Multi Drug Resistance Detection in Pulmonary Tuberculosis Cases in Balochistan. Pak-Euro Journal of Medical and Life Sciences. (2020). Vol 3 No 3. DOI: 10.31580/pjmls.v3i3.1545

19.  Asma Zafar, Muhammad Nauman Aftab, Mushtaq Ahmad Saleem. Pilot-scale production of recombinant hemicellulases and their saccharification potential. Preparative Biochemistry and Biotechnology. (2020), DOI: 10.1080/10826068.2020.1783679 (Impact factor: 1.47).

20.  Irfana Iqbal, Muhammad Nauman Aftab, M. S. Afzal, Asma Zafar, Afshan Kaleem. Characterization of Geobacillus stearothermophilus protease for detergent industry. Revista Mexicana de Ingeniería Química. (2020). Vol. 19, Sup. 1. pp 267-279. (Impact factor: 1.139)

21.  Asma Zafar, Muhammad Nauman Aftab, Irfana Iqbal, Zia ud Din, Mushtaq Ahmad Saleem. Pilot-scale production of a highly thermostable α -amylase enzyme from Thermotoga petrophila cloned into E. coli and its application as a desizer in textile industry. RSC Advances (2019), Vol. 9, pp. 984 –992 (Impact factor: 2.936)

22.  Asma Zafar, Saima Aftab, Muhammad Nauman Aftab, Hamid Mukhtar, Mushtaq Ahmad Saleem. Purification and characterization of cloned endo 1, 4 β-glucanase gene of Bacillus licheniformis for saccharification of plant biomass. Pakistan Journal of Botany. (2019), 51(3), DOI: 10.30848/PJB2019-3(27) (Impact factor: 0.75)

23.  Umbreen Zafar, Muhammad Kamran Taj, Asma Zafar, Imran Nawaz, Imran Taj. Microbial Characterization of Proteus Mirabilis from Patients Wound Admitted at Bolan Medical Complex Hospital Quetta. Jundishapur Journal of Microbiology. (2019).  12(7):e87963. DOI:10.5812/jjm.87963. (Impact Factor: 1.233)

24.  Tanveer Hussain, Zia ud Din, Muhammad Nauman Aftab, Irfana Iqbal, Asma Zafar. Growth and Feed Conversion Response of Four Female Aseel Chicken Varieties to Antibiotic and Probiotic. Pakistan Journal of Zoology, (2019) Vol. 52, Iss. 1, pp 15-21 (Impact factor: 0.547).

25.  Asma Zafar, Moniba Khawar, Mahnoor Chaudhry, Sayyeda Sana Batool, Amjad Hussain & Mushtaq Ahmad Saleem. Azurin: A hard-Core Anticancer Agent. 2019. Biologia (Pakistan)

26.  Asma Zafar, Muhammad N. Aftab, Asma Manzoor, Irfana Iqbal, Zia ud Din, Afshan Kaleem, Atteque ur RahmAn Koharoo and Mushtaq Ahmad Saleem. Purification and Characterization of a recombinant β-Xylosidase from Bacillus licheniformis ATCC 14580 into E. coli Bl21. Pakistan Journal of Pharmaceutical Sciences. (2018). Vol.31, No.6(Suppl), pp.2755-2762 (Impact factor: 0.807)

27.  Irfana Iqbal, Pakeeza Tanweer, Farkhanda Manzoor, Muhammad Nauman Aftab, Afshan Kaleem, Roheena Abdullah, Asma Zafar & Mehwish Iqtedar. Antimicrobial properties of Dalbergia, Brassica and Trifolium honey against burn Microorganism. 2018. Biologia (Pakistan). 64 (II), 281-287.

28.  Muhammad N. Aftab, Asma Zafar, , Iqbal I, Kaleem A, Zia KM and Awan AR. Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis. Bioengineered. (2017). DOI: 10.1080/21655979.2017.1373918. (Impact factor: 1.691)

29.  Muhammad N. Aftab, Asma Zafar & Ali R. Awan. Expression of thermostable β-xylosidase in Escherichia coli for use in saccharification of plant biomass, Bioengineered. (2017). DOI: 10.1080/21655979.2016.1267884 (Impact factor: 1.870)

1. Zoltan Kis, Tania Rodin, Asma Zafar, Zhangxing Lai, Grace Freke, Oliver Fleck, Armando del rio Hernandez, Leila Towhidi, Ryan Pedrigi and Takayuki Homma. Development of a synthetic gene network to modulate gene expression by mechanical forces. (2016). Scientific reports. 6: 29643. DOI: 10.1038/srep29643. www.nature.com/scientific reports/   (Impact factor: 5.228)
2. Asma Zafar, Muhammad Nauman Aftab, Zia ud Din, Saima Aftab, Irfana Iqbal and Ikram ul Haq. Cloning, purification and characterization of highly thermostable gene of Thermotoga petrophila into Escherishia coli. (2015). App. Biochem. Biotechnol. DOI 10.1007/s12010-015-1912-8 (Impact factor: 1.735)
3. Asma Zafar, Muhammad Nauman Aftab, Zia ud Din, Saima Aftab, Irfana Iqbal, Anam Shahid, Arifa Tahir and Ikram ul Haq. Cloning, Expression and purification of xylanase gene from Bacillus licheniformis for use in saccharification of plant biomass. (2015). App. Biochem. Biotechnol. DOI 10.1007/s12010-015-1872-z (Impact factor: 1.735)
4. Irfana Iqbal, Muhammad Nauman Aftab, Mohammed Afzal, Asad Ur-Rehman, Saima Aftab, Asma Zafar, Zia Ud-Din, Ateeq ue Rahman Khuharo, Jawad Iqbal and Ikram Ul-Haq. Purification and characterization of cloned alkaline protease gene of Geobacillus stearothermophilus. (2014) J. Basic Microbiol., 54, pp. 1–12. (Impact factor: 1.823)
5. Saima Aftab, Muhammad Nauman Aftab, Ikram-Ul-Haq, Muhammad Mohsin Javed, Asma Zafar and Irfana Iqbal. Cloning and expression of endo-1 4–β-glucanase gene from Bacillus licheniformis ATCC 14580 into Escherichia coli BL21 (DE 3). (2012). African Journal of Biotechnology. Vol. 11 (12), pp. 2846-2854 (Impact factor: 0.5)

1. Attended 6^th International and 15^th National Conference on Dynamic Trends in Plant Science-Fostering Environment and Food Security, held on May, 9-11, 2017, jointly organized by the Sardar Bahadur Khan University, Quetta, Pakistan and Pakistan Botanical Society at Quetta, Pakistan and participated in oral presentation entitled “Purification and Characterization of cloned β-xylosidase enzyme from Bacillus licheniformis ATCC14580 into coli ”.
2. Participated in two days National Conference on “Defeating the Public Enemy, The Mosquito: A real Challenge” (Poster Presentation) held on 3 – 4 December, 2014, organized by Dengue Prevention and Control Cell, Lahore College for Women University, Lahore.
3. Attended two days training workshop on “Fermenter Studies and End Product Analysis by HPLC”, held in June 29-30, 2015 at Institute of Industrial Biotechnology, GCU, Lahore.
4. Attended 2^nd International Conference of Biotechnology for Sustainable Development, held in November 26-28, 2014 at Institute of Industrial Biotechnology, GCU, Lahore and participated in oral presentation entitled “Fermenter studies of cloned thermostable Alpha amylase gene from Thermotoga peterophila and application in textile industry”.
5. Attended one day seminar on “Biochemistry, Molecular Biology and Bioinformatics (BMB)” arranged by Institute of Industrial Biotechnology, GC University Lahore, Pakistan, on 23^rd October 2014
6. Attended 2^nd International Conference of Plant Scientists and 11^th international meeting of Plant scientists held in February 22-14, 2011 at Biology Department GCU, Lahore, Pakistan organized by Pakistan Botanical Society
7. Participation in poster competition held at Biotechnology Exhibition and Seminar 2010 on 20-21 October, 2010 organized by Institute of Biochemistry and Biotechnology University of Veterinary and Animal Sciences, Lahore, Pakistan
8. Attended Pakistan Botanic Garden Conference on “Action Plan for Botanic Gardens network in Pakistan” held in March 24-25, 2006 at GCU, Lahore, Pakistan organized by Botanic Garden GCU, Lahore, Pakistan

1. Availed HEC IRSIP (International Research Support Initiative Program) Scholarship for six months for research in Bioengineering Department at Imperial College London, 2013
2. Availed Indigenous scholarship entitled “Indigenous Ph.D. Fellowship Program” By Higher Education Commission of Pakistan, 2012.

Four gene Bank Submissions

1. KT427375
2. KT427376
3. KT427377
4. KT378295

One year research experience as a Research officer in a project of Cloning, Expression and characterization of alpha amylase genes from Thermotoga petrophila at institute of Industrial Biotechnology, Govt. College University, Lahore funded by Higher Education Commission, Pakistan
One year research experience as a Research officer in a project of Biofuels production from plant    Biomass at institute of Industrial Biotechnology, Govt. College University, Lahore funded by Ministry of Science and Technology, Pakistan
Two years teaching experience as Science teacher at Al farooq educational system. Al-faisal Town Lahore
One year teaching experience at Al-badar girls High School, Gujranwala as Senior Science teacher
Worked as teaching coordinator for one semester at IIB, GCU, Lahore
Three months research experience as an internee under National Internship Program of the Govt. of Pakistan, in Food labs Saman Abad Lahore

Research interests are in the fields of

• Biotechnology
• Molecular Biology
• Microbiology
• Botany

My objective is to compliment and utilize my abilities for constructive purpose in any prestigious and reputable institute and to avail the opportunity of gaining valuable scientific knowledge experience.

• Dr. Ikram-ul-Haq SI, FAPS
  Professor of emeritus
  Institute of Industrial Biotechnology
  GC University, Lahore
  ikmhaq@yahoo.com
• Dr. Muhammad Nauman Aftab
  Associate Professor
  Institute of Industrial Biotechnology
  GC University, Lahore.
  nauman535@yahoo.com