Course Objectives:

The main objectives of this course are to introduce the students to the basics principles, instrumental aspects and applications of separation and spectrophotometric analytical methods

Course Contents:


Separation Methods:

Principle of solvent extraction, solvent extraction of metals, analytical separations, multiple batch extraction, counter current distribution, solid-phase extraction, solvent extraction by flow injection method, principles of chromatography, classification of chromatographic techniques, overview of paper, thin layer, column, ion exchange chromatography and electrophoresis.

Analytical Spectrophotometry:

Properties of light and its interaction with matter, relation between frequency, velocity and wave number, Lambert- Beer’s law and its limitations, single beam and double beam spectrophotometers, lamps and lasers as sources of light, monochromators, detectors, photomultiplier tube, photodiode array, charged coupled device, FT-IR spectroscopy, fourier analysis, interferometry, noise and its control.

CHEM-211 Lab:

Separation of phenol from given organic mixture using solvent extraction. Separation of given mixture of cations using Paper Chromatography. Analysis of the composition of a mixture of nitro anilines by TLC. Separation of sugars using paper chromatography.
Separation of amino acids using paper/thin layer chromatography. Deionization and softening of water using ion exchange chromatography. Determination of λmax of KMnO4 and K2Cr2O7 solutions and verification of Beer- Lambert’s law.
Determination of stoichiometry of a metal complex by visible spectrometry. Determination of aspirin and caffeine in a proprietary analgesic by double beam UV-Vis. spectrometer.
Quantification of iron in a given sample by using single beam spectrophotometer.
A study of characteristics infrared absorption frequencies.