The main objectives of this course are to introduce the students to the basics principles, instrumental aspects and applications of separation and spectrophotometric analytical methods
Principle of solvent extraction, solvent extraction of metals, analytical separations, multiple batch extraction, counter current distribution, solid-phase extraction, solvent extraction by flow injection method, principles of chromatography, classification of chromatographic techniques, overview of paper, thin layer, column, ion exchange chromatography and electrophoresis.
Properties of light and its interaction with matter, relation between frequency, velocity and wave number, Lambert- Beer’s law and its limitations, single beam and double beam spectrophotometers, lamps and lasers as sources of light, monochromators, detectors, photomultiplier tube, photodiode array, charged coupled device, FT-IR spectroscopy, fourier analysis, interferometry, noise and its control.
Separation of phenol from given organic mixture using solvent extraction. Separation of given mixture of cations using Paper Chromatography. Analysis of the composition of a mixture of nitro anilines by TLC. Separation of sugars using paper chromatography.
Separation of amino acids using paper/thin layer chromatography. Deionization and softening of water using ion exchange chromatography. Determination of λmax of KMnO4 and K2Cr2O7 solutions and verification of Beer- Lambert’s law.
Determination of stoichiometry of a metal complex by visible spectrometry. Determination of aspirin and caffeine in a proprietary analgesic by double beam UV-Vis. spectrometer.
Quantification of iron in a given sample by using single beam spectrophotometer.
A study of characteristics infrared absorption frequencies.